An insight on the <i>N</i>‐glycome of notochordal cell‐rich porcine nucleus pulposus during maturation
نویسندگان
چکیده
Degeneration of the intervertebral disc is an age-related condition. It also accompanies disappearance notochordal cells, which are remnants developmental stages nucleus pulposus (NP). Molecular changes such as extracellular matrix catabolism, cellular phenotype, and glycosaminoglycan loss in NP have been extensively studied. However, one most significant co- posttranslational modifications, glycosylation has overlooked cells degeneration. Here, we aim to characterize N-glycome young mature identify patterns related aging. Accordingly, isolated N-glycans from cell-rich porcine discs, characterized them using a combined approach exoglycosidase digestions analysis with hydrophilic interaction ultra-performance liquid chromatography mass spectrometry. We assigned over 300 individual for each age group. Moreover, observed notable abundance antennary structures, galactosylation, fucosylation, sialylation both groups. In addition, indicated our results, increasing outer arm fucosylation decreasing α(2,3)-linked aging suggest that these traits age-dependent. Lastly, focused on extensive characterization without inferred degeneration, describing specific only. Our findings combination those other studies, degeneration does not involve identical processes
منابع مشابه
the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
Notochordal cell rich nucleus pulposus tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human MSCs
pulposus phenotype in human MSCs +Purmessur, D; Godburn K; Abbott, RD: Schek RM; Iatridis JC +University of Vermont, Burlington VT Senior author [email protected] INTRODUCTION Mesenchymal stem cells (MSCs) are an attractive cell source for IVD repair, yet the means of differentiating these cells to a nucleus pulposus (NP) phenotype is not clear and an area of high priority. Notochordal cel...
متن کاملThe Regenerative Potential of Notochordal Cells in a Nucleus Pulposus Explant
STUDY DESIGN In vitro disk explant culture. OBJECTIVE Notochordal cells (NCs) have been shown to upregulate matrix production by nucleus pulposus (NP) cells in coculture. To examine the translation of these in vitro results to a nativelike setting, the regenerative potential of NCs injected into NP tissue was assessed in this study. METHODS NP explants were cultured after injection with NCs...
متن کاملNotochordal-cell derived extracellular vesicles exert regenerative effects on canine and human nucleus pulposus cells
During intervertebral disc ageing, chondrocyte-like cells (CLCs) replace notochordal cells (NCs). NCs have been shown to induce regenerative effects in CLCs. Since vesicles released by NCs may be responsible for these effects, we characterized NC-derived extracellular vesicles (EVs) and determined their effect on CLCs. EVs were purified from porcine NC-conditioned medium (NCCM) through size exc...
متن کاملChordoma‐derived cell line U‐CH1‐N recapitulates the biological properties of notochordal nucleus pulposus cells
Intervertebral disc degeneration proceeds with age and is one of the major causes of lumbar pain and degenerative lumbar spine diseases. However, studies in the field of intervertebral disc biology have been hampered by the lack of reliable cell lines that can be used for in vitro assays. In this study, we show that a chordoma-derived cell line U-CH1-N cells highly express the nucleus pulposus ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FASEB bioAdvances
سال: 2023
ISSN: ['2573-9832']
DOI: https://doi.org/10.1096/fba.2023-00011